Attempt to isolate infectious agent from bone-marrow of patients with multiple sclerosis.

نویسندگان

  • W C Wallen
  • J L Sever
  • D E McFarlin
  • H F McFarland
  • R G Traub
  • B Rentier
  • J I Greenstein
  • P M Moore
چکیده

Bone-marrow was aspirated into 10 ml Eagle's minimal essential medium (EMEM) containing preservative-free heparin. After washing, the samples were divided into four parts; A for tube cultures,. B for coverslip cultures in multidish wells; C was placed on a 'Ficoll'-'Hypaque' gradient to obtain free mononuclear cells, and D was frozen in liquid nitrogen in cryopreservative medium containing 1% dimethyl sulphoxide.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bone Marrow Transplantation and Medical Tourism at Erciyes University - A Single Center Experience

Medical tourism emerged when people were challenged with high costs and slow service in medical care. As a consequence of these issues, people started to look for medical treatment in other countries. Globalization accelerated the growth of medical tourism. Several “push” and “pull” factors play roles in the motivation of patients to become a medical tourist. This review addresses the different...

متن کامل

In vitro Effect of Pomalidomide on Bone Marrow Mononuclear Cells from Multiple Myeloma Patients

Background: Many features of anticancer drugs, including cytotoxicity and/or cytokine induction, are studied using cell lines orhuman blood leukocytes. However, in a disease such as multiple myeloma, most cancerous cells are resided within bone marrowmononuclear cells. In the present study, we investigated the effect of pomalidomide on apoptosis and IL-2 production of bonemarrow mononuclear cel...

متن کامل

Effect of Multiple Intraperitoneal Injections of Human Bone Marrow Mesenchymal Stem Cells on Cuprizone Model of Multiple Sclerosis

Background: Bone marrow mesenchymal stem cells (BM-MSCs) elicit neuroprotective effects, and their repair ability has been investigated in different experimental models. We aimed to investigate the effect of multiple i.p. BM-MSCs injections in the cuprizone model of multiple sclerosis in mice. Methods: Adult male C57BL/6 mice (n = 40) were fed a regular diet or a diet containing cuprizone (0.2%...

متن کامل

P 28: Bone Marrow-Derived Mesenchymal Stem Cells Reduces Neuroinflammation and Splenic Cytolytic CD8 + T Cells in Mice with Experimental Autoimmune Encephalomyelitis

Introduction: Multiple sclerosis (MS) has been recognized as a common neurodegenerative disease that occurs after an Auto reactive T cells against myelin antigens.  Demyelination and inflammation are the main features of this disease. The anti-inflammatory and neuroprotective roles of bone marrow-derived mesenchymal stem cells (BM-MSCs) have been considered as a suitable tre...

متن کامل

Development of 166Ho-zoledronate as a bone marrow ablative agent

In this study, production, quality control and biodistribution studies of 166Ho-zoledronate have been presented as a possible bone marrow ablative agent. Ho-166 chloride was produced by thermal neutron irradiation of natural 165Ho(NO3)3 samples. 166Ho-zoledronate complex was prepared by adding the desired amount of zoledronate solution (0.2 mL, 150 mg/ml in 1 M NaOH) to appropriate amount of th...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Lancet

دوره 2 8139  شماره 

صفحات  -

تاریخ انتشار 1979